5VU6
TNA polymerase binary complex with primer/template duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 66.090, 110.800, 149.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.928 - 3.000 |
R-factor | 0.2382 |
Rwork | 0.235 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k8z |
RMSD bond length | 0.005 |
RMSD bond angle | 0.748 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.930 | 3.120 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.091 | 0.779 |
Number of reflections | 22536 | |
<I/σ(I)> | 5.8 | |
Completeness [%] | 99.7 | |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M MES, pH 6.0, 16% PEG3500, 0.2 M sodium sulfate, Silver Bullet Bio Additive #56 |