5VU3
Crystal structure of the competence-damaged protein (CinA) superfamily protein ECL_02051 from Enterobacter cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-02-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.529, 52.825, 132.156 |
| Unit cell angles | 85.27, 87.19, 68.98 |
Refinement procedure
| Resolution | 29.867 - 1.868 |
| R-factor | 0.1521 |
| Rwork | 0.151 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kvk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.697 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rpim | 0.040 | 0.379 |
| Number of reflections | 96710 | 4175 |
| <I/σ(I)> | 26.33 | 2.63 |
| Completeness [%] | 93.0 | 90.4 |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | PEG 2K MME, 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 10 mM nicotinamide mononucleotide |
