5VTV

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant in complex with 3'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0331
Spacegroup name	C 1 2 1
Unit cell lengths	208.646, 131.353, 72.780
Unit cell angles	90.00, 97.76, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.18478
Rwork	0.183
R-free	0.21209
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnk
RMSD bond length	0.010
RMSD bond angle	1.481
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.250	2.250
Rpim	0.050	0.380
Number of reflections	92558	8215
<I/σ(I)>	22	1.4
Completeness [%]	99.1	97.2
Redundancy	5.7	4.4
CC(1/2)	1.000	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M sodium cacodylate pH 6.5, 5% PEG 8000, and 38% 2-methyl-2,4-pentanediol