5VTU

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225L/L226S mutant apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0331
Spacegroup name	C 1 2 1
Unit cell lengths	205.554, 129.392, 72.894
Unit cell angles	90.00, 102.66, 90.00

Refinement procedure

Resolution	50.000 - 2.450
R-factor	0.19913
Rwork	0.198
R-free	0.21956
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnk
RMSD bond length	0.009
RMSD bond angle	1.433
Data reduction software	HKL-2000 (712)
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.450	2.450
Rpim	0.040	0.260
Number of reflections	66821	5155
<I/σ(I)>	18.2	1.6
Completeness [%]	98.0	83.3
Redundancy	6.3	4.4
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium nitrate and 20% PEG 3350