5VTQ

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225L/L226S mutant in complex with 3'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0331
Spacegroup name	C 1 2 1
Unit cell lengths	209.960, 131.235, 72.250
Unit cell angles	90.00, 98.62, 90.00

Refinement procedure

Resolution	50.000 - 2.950
R-factor	0.20305
Rwork	0.202
R-free	0.22776
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnk
RMSD bond length	0.008
RMSD bond angle	1.282
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.950	2.950
Rpim	0.090	0.370
Number of reflections	41818	3661
<I/σ(I)>	7.7	1.4
Completeness [%]	99.0	95.2
Redundancy	4.2	3.3
CC(1/2)	0.980	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium cacodylate pH 6.5, 5% PEG 8000, and 38% 2-methyl-2,4-pentanediol