5VTM
The crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.546, 76.760, 102.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.510 - 2.041 |
R-factor | 0.1945 |
Rwork | 0.191 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bw0 3ci0 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.915 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.510 |
High resolution limit [Å] | 2.000 |
Rmeas | 0.052 |
Rpim | 0.015 |
Number of reflections | 31625 |
<I/σ(I)> | 10.3 |
Completeness [%] | 99.2 |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 293 | 19 %-22 % PEG 2000 MME, 0.1M Tris, pH 8.0-9.0, 0.2 M trimethylamine N-oxide |