5VTL
Structure of metacyclic invariant surface protein , Tb427.07.360, from Trypanosoma brucei.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.810, 79.500, 108.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.720 - 1.823 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y44 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.139 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 136043 | 19605 |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% PEG 1500 |
