5VSZ

Structure of the Ubl domain of Sacsin mutant L78M

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97873, 0.97921
Spacegroup name	C 2 2 21
Unit cell lengths	52.140, 61.450, 91.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.129 - 2.400
R-factor	0.2215
Rwork	0.218
R-free	0.28420
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.094
Data reduction software	DENZO
Data scaling software	SCALA (3.3.16)
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.785	30.725	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.050	0.498
Rmeas	0.076	0.075	0.534
Rpim	0.020	0.022	0.139
Total number of observations	82884
Number of reflections	5927
<I/σ(I)>	24.8	12.5	1.5
Completeness [%]	98.9	91.8	98.7
Redundancy	14	10.5	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	0.1 M bicine, 0.25 M NaCl, 1 mM lead acetate, 10% glycerol, pH 8.6