Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-10-25 |
| Detector | ADSC HF-4M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.097, 113.097, 64.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.969 - 3.100 |
| R-factor | 0.21467 |
| Rwork | 0.212 |
| R-free | 0.25774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r4d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 16.969 |
| High resolution limit [Å] | 3.098 |
| Number of reflections | 8557 |
| <I/σ(I)> | 9.15 |
| Completeness [%] | 96.3 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-HCl, PEG4000, NaCl |
