5VSR

ABA-mimicking ligand AMF4 in complex with ABA receptor PYL2 and PP2C HAB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-24
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.2544
Spacegroup name	P 21 21 21
Unit cell lengths	60.210, 64.380, 144.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.624 - 2.618
R-factor	0.2415
Rwork	0.239
R-free	0.27510
RMSD bond length	0.004
RMSD bond angle	0.992
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.400	2.760
High resolution limit [Å]	2.620	2.620
Number of reflections	17605
<I/σ(I)>	10.3
Completeness [%]	99.3
Redundancy	5.7
CC(1/2)	0.993	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M Magnesium formate, 20% PEG3350