5VSB

Structure of DUB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	61.970, 73.740, 84.770
Unit cell angles	90.00, 91.43, 90.00

Refinement procedure

Resolution	61.951 - 1.850
R-factor	0.2353
Rwork	0.235
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.462
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.950	73.740	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.094	0.046	1.225
Number of reflections	64979
<I/σ(I)>	8.9
Completeness [%]	99.7	98.6	99.3
Redundancy	3.6	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, NaFormate