5VS5

ABA-mimicking ligand AMF2alpha in complex with ABA receptor PYL2 and PP2C HAB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.978918
Spacegroup name	P 21 21 21
Unit cell lengths	59.770, 64.320, 146.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.283 - 2.800
R-factor	0.236
Rwork	0.232
R-free	0.29320
RMSD bond length	0.008
RMSD bond angle	1.123
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	72.900
High resolution limit [Å]	2.330
Rmerge	0.145
Number of reflections	14604
<I/σ(I)>	9.1
Completeness [%]	99.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M di-Sodium malonate,20% PEG3350