5VRQ
Crystal structure of Legionella pneumophila effector AnkC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.6307 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.705, 78.705, 156.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.689 - 3.205 |
R-factor | 0.2257 |
Rwork | 0.223 |
R-free | 0.28040 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.513 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.318 |
High resolution limit [Å] | 3.205 | 3.205 |
Number of reflections | 8451 | 832 |
<I/σ(I)> | 51.9 | 7.1 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 18.7 | 19.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.25 M magnesium formate, 5 mM EDTA, 0.1 M sodium acetate |