5VRO
ABA-mimicking ligand AMF1beta in complex with ABA receptor PYL2 and PP2C HAB1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-02 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.2544 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.294, 67.940, 145.899 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.546 - 2.257 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.24420 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.009 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.260 |
Rmerge | 0.076 |
Number of reflections | 29406 |
<I/σ(I)> | 73.1 |
Completeness [%] | 99.8 |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2M di-Sodium malonate, 20% PEG3350 |