5VRF
CryoEM Structure of the Zinc Transporter YiiP from helical crystals
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.1 |
| Number of Particle | 141904 |
| Software | SPARX/EMAN2,Coot,RELION,PHENIX,Leginon,CTFFIND,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
