5VR2

mouse myocilin leucine zipper C-terminal 7 heptad repeat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	85.129, 48.746, 55.798
Unit cell angles	90.00, 105.97, 90.00

Refinement procedure

Resolution	41.881 - 1.922
R-factor	0.192
Rwork	0.187
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.745
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	AMPLE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.881	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.118	0.074	0.652
Rmeas	0.129	0.081	0.761
Rpim	0.050	0.033	0.382
Total number of observations	142081
Number of reflections	23662
<I/σ(I)>	6.6
Completeness [%]	97.6	99.3	80.7
Redundancy	6	6.6	2.9
CC(1/2)		0.995	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M ammonium acetate, 0.1 M BisTris pH 5.5, and 17% PEG 10,000