5VR0
Crystal structure of glucose isomerase from Streptomyces rubiginosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-01 |
Detector | SBC-3 |
Wavelength(s) | 0.9792, 1.5406 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.854, 98.061, 102.279 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.900 - 1.700 |
R-factor | 0.11988 |
Rwork | 0.119 |
R-free | 0.14161 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1xib |
RMSD bond length | 0.007 |
RMSD bond angle | 1.333 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.010 |
High resolution limit [Å] | 1.700 |
Rpim | 0.013 |
Number of reflections | 49609 |
<I/σ(I)> | 125 |
Completeness [%] | 96.2 |
Redundancy | 43.1 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M CaCl2, 16-22% MPD (v/v) and 0.1 M Tris-HCl pH 7.0 |