5VQI
Nuclear transport of the Neurospora crassa NIT2 transcription factor is mediated by Importin-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-30 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1,45 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.034, 63.706, 183.635 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.909 - 2.501 |
R-factor | 0.1828 |
Rwork | 0.179 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rxh |
RMSD bond length | 0.008 |
RMSD bond angle | 1.196 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.910 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.263 |
Number of reflections | 18973 |
<I/σ(I)> | 5.1 |
Completeness [%] | 99.7 |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2 mM Bicine pH 8.5 and 20% (w/v) polyethylene glycol 6000 |