5VQG
Crystal structure of the extended Tudor domain from BmPAPI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-01-13 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 63.560, 63.560, 100.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.780 - 2.600 |
R-factor | 0.20571 |
Rwork | 0.201 |
R-free | 0.27705 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fdr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.416 |
Data reduction software | iMOSFLM |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.780 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.106 | 0.608 |
Number of reflections | 7535 | 915 |
<I/σ(I)> | 7.8 | 1.6 |
Completeness [%] | 99.5 | 100 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281 | 1% PEG 8,000, 50 mM MES, pH 5.5, 100 mM KCl and 10 mM MgCl2 |