5VQG
Crystal structure of the extended Tudor domain from BmPAPI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-01-13 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.560, 63.560, 100.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.780 - 2.600 |
| R-factor | 0.20571 |
| Rwork | 0.201 |
| R-free | 0.27705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fdr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.416 |
| Data reduction software | iMOSFLM |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.780 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.106 | 0.608 |
| Number of reflections | 7535 | 915 |
| <I/σ(I)> | 7.8 | 1.6 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 1% PEG 8,000, 50 mM MES, pH 5.5, 100 mM KCl and 10 mM MgCl2 |
