5VQB
Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-04-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.085, 129.285, 75.311 |
| Unit cell angles | 90.00, 105.52, 90.00 |
Refinement procedure
| Resolution | 48.920 - 3.391 |
| R-factor | 0.2284 |
| Rwork | 0.226 |
| R-free | 0.28360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kow |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.447 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 3.460 |
| High resolution limit [Å] | 3.391 | 3.400 |
| Rpim | 0.173 | 0.565 |
| Number of reflections | 25915 | 1305 |
| <I/σ(I)> | 5.7 | 1.68 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.457 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1 M MES pH 6, 0.2 M ammonium sulfate, 25% PEG 5K |
