5VQ9

Structure of human TRIP13, Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	96.964, 96.964, 122.646
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.973 - 3.020
R-factor	0.2009
Rwork	0.199
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xgu
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.650	3.200
High resolution limit [Å]	3.020	3.020
Rmerge	0.087	1.116
Rpim	0.040	0.514
Number of reflections	12908	2069
<I/σ(I)>	19	1.8
Completeness [%]	100.0	100
Redundancy	5.7	5.7
CC(1/2)	0.999	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl pH 8.5, 0.8 M LiCl, 4% PEG 3350, cryoprotected with 20% glycerol