5VQ5
Crystal Structure of the Lectin Domain From the F17-like Adhesin, UclD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-05-16 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.010, 58.600, 175.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.570 - 1.600 |
R-factor | 0.1739 |
Rwork | 0.172 |
R-free | 0.21810 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.582 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.570 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.053 | 0.430 |
Number of reflections | 108142 | |
<I/σ(I)> | 17.68 | 1.43 |
Completeness [%] | 99.9 | 99.98 |
Redundancy | 2 | 2 |
CC(1/2) | 0.659 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.1 M KH2PO4, 0.2 M KI |