5VPE
Transcription factor FosB/JunD bZIP domain in complex with cognate DNA, type-I crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.479, 90.755, 105.618 |
| Unit cell angles | 90.00, 117.93, 90.00 |
Refinement procedure
| Resolution | 47.307 - 2.053 |
| R-factor | 0.2191 |
| Rwork | 0.218 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fos |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.747 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.100 |
| High resolution limit [Å] | 2.060 | 5.590 | 2.060 |
| Rmerge | 0.055 | 0.043 | 0.931 |
| Rmeas | 0.060 | 0.047 | 1.022 |
| Rpim | 0.024 | 0.019 | 0.414 |
| Total number of observations | 357830 | ||
| Number of reflections | 58932 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 98.3 | 98.2 | 98.2 |
| Redundancy | 6.1 | 6.4 | 5.9 |
| CC(1/2) | 0.995 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.9 M Na2HPO4 and 24% (v/v) ethylene glycol |
