5VPD
Transcription factor FosB/JunD bZIP domain in its oxidized form, type-III crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.008, 98.051, 50.251 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.720 - 2.790 |
R-factor | 0.2547 |
Rwork | 0.249 |
R-free | 0.30020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vpa |
RMSD bond length | 0.003 |
RMSD bond angle | 0.585 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.720 | 44.720 | 2.820 |
High resolution limit [Å] | 2.790 | 7.510 | 2.790 |
Rmerge | 0.108 | 0.033 | 0.906 |
Rmeas | 0.116 | 0.037 | 0.977 |
Rpim | 0.043 | 0.015 | 0.363 |
Number of reflections | 11778 | ||
<I/σ(I)> | 7.5 | 2 | |
Completeness [%] | 99.9 | 98.4 | 100 |
Redundancy | 7.1 | 6.2 | 7.1 |
CC(1/2) | 0.999 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.1 M NaCl, 50 mM sodium cacodylate pH 6 and 10 mM spermine |