5VOO
Methionine synthase folate-binding domain with methyltetrahydrofolate from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 188.463, 188.463, 247.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 163.210 - 2.400 |
| R-factor | 0.19235 |
| Rwork | 0.190 |
| R-free | 0.22972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5von |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.363 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 163.210 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.092 | 0.645 |
| Rpim | 0.048 | 0.342 |
| Number of reflections | 100615 | 4857 |
| <I/σ(I)> | 8.93 | 1.92 |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 4.5 | 4.4 |
| CC(1/2) | 0.996 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M sodium citrate pH 4.5, 22 % polyethylene glycol 3350, 0.2 M tri-sodium citrate, soaked with 6S-methyltetrahydrofolate |
