5VON
Methionine synthase folate-binding domain from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.186, 55.186, 197.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.140 - 2.100 |
| R-factor | 0.20519 |
| Rwork | 0.202 |
| R-free | 0.25933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bof |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.297 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.140 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.329 |
| Number of reflections | 18533 | 1803 |
| <I/σ(I)> | 14.74 | 1.96 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 6.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8 M Succinic acid |
