5VNR

X-ray structure of perdeuterated T4 lysozyme cysteine-free pseudo-wild type at cryogenic temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-04-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	59.830, 59.830, 95.461
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.546 - 1.631
R-factor	0.1604
Rwork	0.158
R-free	0.18560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lw9
RMSD bond length	0.006
RMSD bond angle	0.790
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.630
Rmerge	0.096
Number of reflections	25255
<I/σ(I)>	20.1
Completeness [%]	99.7
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	~2.0 M Na/K phosphate, pH 6-7, 250 mM NaCl, 40mM 2-hydroxyethyl disulfide