5VNQ
Neutron crystallographic structure of perdeuterated T4 lysozyme cysteine-free pseudo-wild type at cryogenic temperature
Experimental procedure
Experimental method | LAUE |
Source type | NUCLEAR REACTOR |
Source details | ORNL High Flux Isotope Reactor BEAMLINE CG4D |
Synchrotron site | ORNL High Flux Isotope Reactor |
Beamline | CG4D |
Temperature [K] | 80 |
Detector technology | IMAGE PLATE |
Collection date | 2016-10-04 |
Detector | FUJI |
Wavelength(s) | 2.8 - 4.6 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.500, 61.500, 95.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.700 - 2.200 |
R-factor | 0.2431 |
Rwork | 0.242 |
R-free | 0.28020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lw9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.658 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.700 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.162 | 0.214 |
Number of reflections | 7965 | |
<I/σ(I)> | 6.5 | |
Completeness [%] | 72.7 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | ~2.0 M Na/K phosphate, pH 6-7, 250 mM NaCl, 40mM 2-hydroxyethyl disulfide |