5VN9

Structure of bacteriorhodopsin from crystals grown at 4 deg C using GlyNCOC15+4 as an LCP host lipid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	50.070, 109.630, 56.560
Unit cell angles	90.00, 116.27, 90.00

Refinement procedure

Resolution	28.342 - 2.594
R-factor	0.2386
Rwork	0.223
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xji
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	XDS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.342	2.690
High resolution limit [Å]	2.590	2.590
Rmerge	0.143	0.317
Number of reflections	15034	1396
<I/σ(I)>	5.3	1.7
Completeness [%]	90.0	50
Redundancy	2.7	1.7
CC(1/2)	0.987	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	277	1.9-3.0 M sodium/potassium phosphate buffer (pH5.6), 3.5% v/v methylpentanediol and 0.5% w/v OG