5VMW

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a double CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 2
Unit cell lengths	44.341, 184.149, 105.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.669 - 2.397
R-factor	0.1805
Rwork	0.176
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6n
RMSD bond length	0.011
RMSD bond angle	1.205
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.669	2.480
High resolution limit [Å]	2.397	2.400
Rmerge	0.148	0.634
Number of reflections	17193	1540
<I/σ(I)>	12.8	2.04
Completeness [%]	98.4	88.81
Redundancy	9.5	4.2
CC(1/2)	0.997	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.1 M Disodium tartrate, 20 % PEG 3350