5VMW
Kaiso (ZBTB33) zinc finger DNA binding domain in complex with a double CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 44.341, 184.149, 105.189 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.669 - 2.397 |
R-factor | 0.1805 |
Rwork | 0.176 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6n |
RMSD bond length | 0.011 |
RMSD bond angle | 1.205 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.669 | 2.480 |
High resolution limit [Å] | 2.397 | 2.400 |
Rmerge | 0.148 | 0.634 |
Number of reflections | 17193 | 1540 |
<I/σ(I)> | 12.8 | 2.04 |
Completeness [%] | 98.4 | 88.81 |
Redundancy | 9.5 | 4.2 |
CC(1/2) | 0.997 | 0.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | 0.1 M Disodium tartrate, 20 % PEG 3350 |