5VMV
Kaiso (ZBTB33) zinc finger DNA binding domain in complex with its double CpG-methylated DNA consensus binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 44.219, 183.792, 104.566 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.443 - 2.313 |
R-factor | 0.2075 |
Rwork | 0.203 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6n |
RMSD bond length | 0.011 |
RMSD bond angle | 1.203 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.443 | 2.396 |
High resolution limit [Å] | 2.313 | 2.313 |
Rmerge | 0.086 | 0.277 |
Number of reflections | 17383 | 946 |
<I/σ(I)> | 13.11 | 4.29 |
Completeness [%] | 90.8 | 51.16 |
Redundancy | 6 | 3.6 |
CC(1/2) | 0.996 | 0.945 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris pH 8.5, 0.2 M Ammonium Acetate, 30 % (v/v) 2-propanol |