5VMJ
Influenza hemagglutinin H1 mutant DH1E in complex with 3'SLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-11-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.940, 81.050, 120.950 |
Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
Resolution | 54.825 - 2.950 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wrg |
RMSD bond length | 0.004 |
RMSD bond angle | 0.888 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.380 |
High resolution limit [Å] | 2.950 |
Number of reflections | 39386 |
<I/σ(I)> | 6.9 |
Completeness [%] | 100.0 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.20 M NaF, 18% PEG3350 |