5VMD
Crystal structure of UBR-box from UBR6 in a domain-swapping conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-23 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.2822 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.570, 69.200, 82.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.720 - 2.202 |
R-factor | 0.1974 |
Rwork | 0.195 |
R-free | 0.24740 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.684 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.720 | 2.281 |
High resolution limit [Å] | 2.200 | 2.202 |
Rmeas | 0.094 | 0.608 |
Number of reflections | 20183 | 1952 |
<I/σ(I)> | 14.67 | 2.88 |
Completeness [%] | 99.8 | 99 |
Redundancy | 4.3 | 4.1 |
CC(1/2) | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2 M sodium nitrate, 0.1 M Bis-Tris propane, pH 6.5, 25% PEG3350 |