5VM4
The apo form of the triclocarban-binding single domain camelid nanobody VHH T10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.566, 93.254, 110.576 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.19914 |
| Rwork | 0.197 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T10 holo subunit A |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.553 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.260 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.077 | 0.281 |
| Number of reflections | 120758 | |
| <I/σ(I)> | 10 | 4.3 |
| Completeness [%] | 96.6 | 90.8 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | protein concentration: 21 mg/ml well: 1.8305 M malonic acid, 0.25 M ammonium citrate tribasic, 0.12 M succinic acid, 0.3 M DL-malic acid, 0.4 M sodium acetate trihydrate, 0.5 M sodium formate, 0.16 M ammonium tartrate dibasic |
