5VM2
Crystal structure of ECK1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-04 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97860 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 63.994, 70.888, 149.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.312 - 1.983 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.927 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2733) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.106 | 0.582 |
| Rpim | 0.045 | 0.293 |
| Number of reflections | 47841 | 2349 |
| <I/σ(I)> | 22.7 | 2 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 6.3 | 4.7 |
| CC(1/2) | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.2 M sodium chloride, 0.1 M sodium citrate pH 5.6, 1 mM magnesium chloride, 25% (w/v) PEG3350 |
