5VM2
Crystal structure of ECK1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-04 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97860 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 63.994, 70.888, 149.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.312 - 1.983 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.22450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e3j |
RMSD bond length | 0.005 |
RMSD bond angle | 0.927 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2733) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.106 | 0.582 |
Rpim | 0.045 | 0.293 |
Number of reflections | 47841 | 2349 |
<I/σ(I)> | 22.7 | 2 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 6.3 | 4.7 |
CC(1/2) | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.2 M sodium chloride, 0.1 M sodium citrate pH 5.6, 1 mM magnesium chloride, 25% (w/v) PEG3350 |