5VLC
Crystal Structure of Medicago truncatula L-Histidinol Dehydrogenase in Complex with L-Histidinol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-17 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.859, 139.201, 102.692 |
| Unit cell angles | 90.00, 119.18, 90.00 |
Refinement procedure
| Resolution | 48.730 - 1.970 |
| R-factor | 0.17888 |
| Rwork | 0.179 |
| R-free | 0.22912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kae |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 2.090 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.078 | 0.905 |
| Number of reflections | 175983 | 27746 |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 6.14 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 292 | 100 mM MES pH 5.2, 200 mM NACl, 12% PEG 3350 |
