5VLB
Crystal Structure of Medicago truncatula L-Histidinol Dehydrogenase in Complex with Imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-12 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.752, 142.814, 105.357 |
| Unit cell angles | 90.00, 120.16, 90.00 |
Refinement procedure
| Resolution | 38.520 - 2.250 |
| R-factor | 0.18159 |
| Rwork | 0.181 |
| R-free | 0.23388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kae |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.520 | 2.390 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.077 | 0.611 |
| Number of reflections | 120737 | 15117 |
| <I/σ(I)> | 15.7 | 2.06 |
| Completeness [%] | 94.7 | 73.8 |
| Redundancy | 5.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | Molecular Dimensions Morpheus Condition H1: 0.1 M Amino acids, 0.1 M Buffer System 1 pH 6.5, 50 % v/v Precipitant Mix 1 |
