5VKZ
Crystal structure of Mdm12 and combinatorial reconstitution of Mdm12/Mmm1 ERMES complexes for structural studies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 83 |
Detector technology | PIXEL |
Collection date | 2011-04-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 116.020, 116.020, 161.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 85.340 - 4.100 |
R-factor | 0.24308 |
Rwork | 0.242 |
R-free | 0.25379 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gyd |
RMSD bond length | 0.011 |
RMSD bond angle | 1.531 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.300 | 4.210 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.115 | 1.050 |
Number of reflections | 10272 | 744 |
<I/σ(I)> | 9.4 | 2 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 7.4 | 7.5 |
CC(1/2) | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 15-25% PEG 3350, 400 mM ammonium phosphate, 3.5 mM Mega-10 |