5VKL
SPT6 tSH2-RPB1 1476-1500 pS1493
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-11-16 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.014, 103.540, 115.487 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.644 - 2.198 |
R-factor | 0.1835 |
Rwork | 0.177 |
R-free | 0.24330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3psj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.878 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.280 |
High resolution limit [Å] | 2.198 | 4.740 | 2.200 |
Rmerge | 0.055 | 0.035 | 0.445 |
Rmeas | 0.056 | 0.035 | 0.458 |
Rpim | 0.011 | 0.006 | 0.106 |
Number of reflections | 12637 | ||
<I/σ(I)> | 23.8 | ||
Completeness [%] | 93.0 | 99.4 | 84.7 |
Redundancy | 24.7 | 30.9 | 17 |
CC(1/2) | 1.000 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 40% ethanol, 100 mM Tris pH 7.0 |