5VKH

Closed conformation of KcsA Y82A-F103A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-06-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 4
Unit cell lengths	155.737, 155.737, 76.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.907 - 2.250
R-factor	0.1752
Rwork	0.174
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.910
High resolution limit [Å]	2.250
Number of reflections	42773
<I/σ(I)>	5.7
Completeness [%]	98.4
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400 , 50 mM magnesium acetate, 50 mM sodium acetate