5VKD

Crystal structure of C-terminal domain of Ebola (Bundibugyo) nucleoprotein in complex with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2016-04-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.407, 87.485, 130.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.471 - 1.749
R-factor	0.1767
Rwork	0.176
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.649
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.880
High resolution limit [Å]	1.749
Number of reflections	53300
<I/σ(I)>	1.69
Completeness [%]	99.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.2M Ammonium formate, 10% w/v Polyvinylpyrrolidone, 20% w/v PEG 4000