5VJW

Arabidopsis thaliana Rhizobiales-like phosphatase 2 complexed with tungstate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.21416
Spacegroup name	P 61
Unit cell lengths	102.660, 102.660, 60.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.1776
Rwork	0.176
R-free	0.19940
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.098	0.950
Number of reflections	31880	2447
<I/σ(I)>	12.3	1.9
Completeness [%]	99.0	99
Redundancy	5	5
CC(1/2)	0.998	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM Bis-Tris-Cl, 250 g/L PEG 3350, 200 g/L glycerol