5VJV
Rhizobiales-like phosphatase 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-30 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.03318 |
Spacegroup name | P 61 |
Unit cell lengths | 102.600, 102.600, 61.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.950 |
R-factor | 0.1856 |
Rwork | 0.184 |
R-free | 0.21910 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.205 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.109 | 1.150 |
Number of reflections | 25380 | 1958 |
<I/σ(I)> | 12.1 | 1.6 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 5.5 | 5.5 |
CC(1/2) | 0.998 | 0.537 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM Bis-Tris-Cl, 250 g/L PEG 3350, 200 g/L glycerol |