5VJJ
Crystal structure of the flax-rust effector AvrP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-03 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.282, 1.262 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.937, 88.937, 45.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.010 - 2.520 |
R-factor | 0.2235 |
Rwork | 0.222 |
R-free | 0.26420 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.761 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | CRANK |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.010 | 2.650 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmeas | 0.182 | 0.891 |
Rpim | 0.049 | |
Number of reflections | 6602 | |
<I/σ(I)> | 16.6 | 2.5 |
Completeness [%] | 99.8 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Bis-Tris pH 7.0, 22% (w/v) PEG 3350 and 0.2 M NaCl |