5VJI
Crystal structure of the CLOCK Transcription Domain Exon19 in Complex with a Repressor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.339, 77.585, 150.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.040 - 1.860 |
R-factor | 0.2174 |
Rwork | 0.214 |
R-free | 0.28020 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.169 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.890 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.079 | |
Number of reflections | 24841 | |
<I/σ(I)> | 28.6 | |
Completeness [%] | 97.7 | 90.2 |
Redundancy | 9.2 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.3 | 291 | 100mM Bis-tris pH7.3 27% PEG2000MME 1% beat-DDM |