5VJE
Class II fructose-1,6-bisphosphate aldolase of Escherichia coli with D-glucitol 1,6-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-01 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.99 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.250, 71.869, 88.983 |
Unit cell angles | 90.00, 108.43, 90.00 |
Refinement procedure
Resolution | 29.011 - 1.650 |
R-factor | 0.1398 |
Rwork | 0.138 |
R-free | 0.16420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b57 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.824 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.011 |
High resolution limit [Å] | 1.650 |
Number of reflections | 81148 |
<I/σ(I)> | 28.2 |
Completeness [%] | 98.3 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 4000, MgCl2, Hepes buffer |