5VJD
Class II fructose-1,6-bisphosphate aldolase of Escherichia coli with DHAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.174, 71.454, 88.185 |
| Unit cell angles | 90.00, 108.94, 90.00 |
Refinement procedure
| Resolution | 39.871 - 1.701 |
| R-factor | 0.1485 |
| Rwork | 0.147 |
| R-free | 0.17250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b57 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.871 |
| High resolution limit [Å] | 1.701 |
| Number of reflections | 72711 |
| <I/σ(I)> | 19 |
| Completeness [%] | 99.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 4000, MgCl2, Hepes buffer |
