5VIZ

X-Ray structure of Insulin Glargine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.951
Spacegroup name	I 21 3
Unit cell lengths	77.997, 77.997, 77.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.150 - 1.700
R-factor	0.1642
Rwork	0.162
R-free	0.18260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.028
RMSD bond angle	2.236
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.160
High resolution limit [Å]	1.700
Number of reflections	8880
<I/σ(I)>	21.5
Completeness [%]	99.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		291	Microbatch