5VIX
Crystal structure of Polyubiquitin with 3 ub domains, domains 1 and 2, from Naegleria fowleri ATCC 30863
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.020, 67.710, 35.830 |
Unit cell angles | 90.00, 114.49, 90.00 |
Refinement procedure
Resolution | 32.608 - 1.550 |
R-factor | 0.1739 |
Rwork | 0.171 |
R-free | 0.20410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h07 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.937 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.608 | 50.000 | 1.590 |
High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
Rmerge | 0.054 | 0.037 | 0.567 |
Rmeas | 0.061 | 0.044 | 0.653 |
Number of reflections | 21353 | 247 | 1549 |
<I/σ(I)> | 14 | 30.46 | 2.31 |
Completeness [%] | 99.2 | 96.9 | 99.7 |
Redundancy | 4.16 | 3.842 | 4.056 |
CC(1/2) | 0.998 | 0.995 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | Rigaku Reagents JCSG+ C3: 200mM Ammonium nitrate, 20% PEG 3350: NafoA.19251.a.B1.PS38167 at 20mg/ml: cryo: 20% EG: tray 288541C3: puck FIQ0-2. |