5VIV
Crystal structure of monomeric near-infrared fluorescent protein miRFP670
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.539, 53.282, 107.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 1.330 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.18900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xtq |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.380 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.084 | 0.735 |
Number of reflections | 69538 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.6 | 98.6 |
Redundancy | 7.9 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 3.4 M NaCl, 0.08 M Tris-HCl pH 7.5 |